Oral Presentation 2014 Cutaneous Biology Meeting

Cellular Crosstalk in the Microenvironment of Cutaneous T cell Lymphoma (#42)

Christenze Thode 1 , Anders Woetmann 2 , Hans H Wandall 3 , Michael C Carlsson 3 , Klaus Qvortrup 4 , Claudia S Kauczok 5 , Marion Wobser 5 , Andreas Printzlau 6 , Niels Ødum 2 , Sally Dabelsteen 1
  1. Department of Oral Medicine and Pathology, School of Dentistry, University of Copenhagen, Copenhagen, Denmark
  2. Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
  3. Copenhagen Center for Glycomics, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
  4. Department of Biomedical Sciences/CFIM, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
  5. Tumorbiologisches Forschungslabor, , Würzburg, Germany
  6. Bodylift Center, , Virum, Denmark

Cutaneous T cell lymphomas (CTCL) are the most common primary skin lymphomas; which are characterized by an accumulation of malignant T cells in the skin. The early lesion resembles both clinically and histologically benign inflammatory disorders, which also presents with hyperproliferative epidermis and T cell infiltration. Despite considerable progress in understanding the molecular mechanisms involved in the malignant transformation of T cells, the causes of the morphological and histopathological features of the disease are largely unknown. We used a novel organotypic model of CTCL to show that malignant T cells through the secretion of Galectin-1 and -3 stimulate vigorous growth of keratinocytes. In parallel, malignant T cells induce disorganized keratinocyte stratification, resembling the early hyperproliferative stage of CTCL. We also observed a loss of attachment between the epithelial and mesenchymal compartments. In addition, hyperproliferation was followed by a downregulation of differentiation markers, such as keratin 10 and involucrin, and a decrease in barrier formation. In conclusion, we provide evidence that malignant T cells orchestrate the histopathological epidermal changes seen in CTCL